The ββα fold of zinc finger proteins as a “natural” protecting group. Chemoselective synthesis of a DNA-binding zinc finger derivative

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2014_chemcom_rodriguez_fold.pdf (2.34 MB)
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URI: http://hdl.handle.net/10347/18487
ISSN: 1359-7345
E-ISSN: 1364-548X
DOI: 10.1039/C3CC47599A

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2014

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Royal Society of Chemistry
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We report the selective modification of cysteine residues engineered in peptides that have two additional cysteine residues as part of a Cys2His2 zinc finger motif. The chemoselective modification is achieved, thanks to the protecting effect exerted by the zinc cation upon coordination with the native cysteines and histidines of the zinc-finger fold. The strategy allows a straightforward synthesis of DNA binding zinc finger constructs

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Rodríguez, J., Mosquera, J., Vázquez, O., Vázquez, M., & Mascareñas, J. (2014). The ββα fold of zinc finger proteins as a “natural” protecting group. Chemoselective synthesis of a DNA-binding zinc finger derivative. Chem. Commun.,50, 2258-2260. doi: 10.1039/c3cc47599a

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We acknowledge the support from the Spanish grants SAF2010-20822-C02, CTQ2009-14431/BQU, CSD2007-00006, the Xunta de Galicia GRC2010/12, GRC2013-041 and INCITE09 209 084PR. J. R. thanks the Fundación Gil Dávila and the Xunta de Galicia and J. M. the Spanish MCINN for their PhD fellowships

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© The Royal Society of Chemistry 2014

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Química Orgánica