Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

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2017_chemcommun_morris_lanthanide_peptide.pdf (624 KB)
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URI: http://hdl.handle.net/10347/17023
ISSN: 1359-7345
E-ISSN: 1364-548X
DOI: 10.1039/C6CC09948C

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2017

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Royal Society of Chemistry
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We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety

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González-Vera, J., Bouzada, D., Bouclier, C., Eugenio Vázquez, M., & Morris, M. (2017). Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity. Chemical Communications, 53(45), 6109-6112. doi: 10.1039/c6cc09948c

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This work was funded by the CNRS (Centre National de la Recherche Scientifique) and a Marie-Curie fellowship EC-FP7 Framework (PIEF-GA-2013-623151) supporting JAGV. CB was funded by the INCA (PRTK-2014). Financial support from the Spanish MINECO (CTQ2015-70698-R), the Xunta de Galicia (Centro singular de investigacio´n de Galicia accreditation 2016–2019), and the European Union (European Regional Development Fund – ERDF), are gratefully acknowledged

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© The Royal Society of Chemistry 2017

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Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS)
Química Orgánica