RT Journal Article
T1 Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity
A1 González-Vera, Juan A.
A1 Bouzada Reboredo, David
A1 Bouclier, Céline
A1 Vázquez Sentís, Marco Eugenio
A1 Morris, May C.
AB We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety
PB Royal Society of Chemistry
SN 1359-7345
YR 2017
FD 2017
LK http://hdl.handle.net/10347/17023
UL http://hdl.handle.net/10347/17023
LA eng
NO González-Vera, J., Bouzada, D., Bouclier, C., Eugenio Vázquez, M., & Morris, M. (2017). Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity. Chemical Communications, 53(45), 6109-6112. doi: 10.1039/c6cc09948c
NO This work was funded by the CNRS (Centre National de la Recherche Scientifique) and a Marie-Curie fellowship EC-FP7 Framework (PIEF-GA-2013-623151) supporting JAGV. CB was funded by the INCA (PRTK-2014). Financial support from the Spanish MINECO (CTQ2015-70698-R), the Xunta de Galicia (Centro singular de investigacio´n de Galicia accreditation 2016–2019), and the European Union (European Regional Development Fund – ERDF), are gratefully acknowledged
DS Minerva
RD 24 abr 2026