Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

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dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Molecularesgl
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánicagl
dc.contributor.authorGonzález-Vera, Juan A.
dc.contributor.authorBouzada Reboredo, David
dc.contributor.authorBouclier, Céline
dc.contributor.authorVázquez Sentís, Marco Eugenio
dc.contributor.authorMorris, May C.
dc.date.accessioned2018-07-13T12:39:22Z
dc.date.available2018-07-13T12:39:22Z
dc.date.issued2017
dc.description.abstractWe describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moietygl
dc.description.peerreviewedSIgl
dc.description.sponsorshipThis work was funded by the CNRS (Centre National de la Recherche Scientifique) and a Marie-Curie fellowship EC-FP7 Framework (PIEF-GA-2013-623151) supporting JAGV. CB was funded by the INCA (PRTK-2014). Financial support from the Spanish MINECO (CTQ2015-70698-R), the Xunta de Galicia (Centro singular de investigacio´n de Galicia accreditation 2016–2019), and the European Union (European Regional Development Fund – ERDF), are gratefully acknowledgedgl
dc.identifier.citationGonzález-Vera, J., Bouzada, D., Bouclier, C., Eugenio Vázquez, M., & Morris, M. (2017). Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity. Chemical Communications, 53(45), 6109-6112. doi: 10.1039/c6cc09948cgl
dc.identifier.doi10.1039/C6CC09948C
dc.identifier.essn1364-548X
dc.identifier.issn1359-7345
dc.identifier.urihttp://hdl.handle.net/10347/17023
dc.language.isoenggl
dc.publisherRoyal Society of Chemistrygl
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-70698-R/ES/SISTEMAS SUPRAMOLECULARES PARA RECONOCIMIENTO BIOMOLECULAR: HELICATOS PEPTIDICOS Y RECEPTORES OLIGOMERICOS AUTOENSAMBLADOS
dc.relation.publisherversionhttps://doi.org/10.1039/C6CC09948Cgl
dc.rights© The Royal Society of Chemistry 2017gl
dc.rights.accessRightsopen accessgl
dc.titleLanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activitygl
dc.typejournal articlegl
dc.type.hasVersionAMgl
dspace.entity.typePublication
relation.isAuthorOfPublication34f1e1e8-411b-4b42-a25c-253481d3abef
relation.isAuthorOfPublication.latestForDiscovery34f1e1e8-411b-4b42-a25c-253481d3abef

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