Selective DNA-Binding by Designed Bisbenzamidine-Homeodomain Chimeras

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Selective_DNA_Binding_by_Designed_Bisbenzamidine_Homeodomain_Chimeras.pdf (756.51 KB)
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URI: http://hdl.handle.net/10347/11967
ISSN: 1439-7633
DOI: 10.1002/cbic.201400079

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2014-04-24

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WILEY-VCH Verlag GmbH
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Abstract

We report the construction of conjugates between three variants of the helix 3 region of a Q50K engrailed homeodomain and bisbenzamidine minor-groove DNA binders. The hybrid featuring the sequence of the native protein failed to bind to DNA; however, modifications that increased the α-helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction

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DNA recognition| Molecular recognition| Oligonucleotides| Peptides| Supramolecular chemistry

Bibliographic citation

Mosquera, J., Rodríguez, J., Vázquez, M. E. and Mascareñas, J. L. (2014), Selective DNA-Binding by Designed Bisbenzamidine-Homeodomain Chimeras. ChemBioChem, 15: 1092–1095. doi: 10.1002/cbic.201400079

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Ministerio de Economía y Competitividad. Grant Numbers: SAF2010-20822-C02, CTQ2012-31341 Consolider Ingenio. Grant Number: 2010 CSD2007-00006 Xunta de Galicia. Grant Number: GRC2013/14 European Regional Development Fund European Research Council. Grant Number: Na 340055 Ministerio de Ciencia e Innovación Fundación Gil Dávila

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© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

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Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS)
Química Orgánica