Interspecies differences in protein expression do not impact the spatiotemporal regulation of glycoprotein VI mediated activation

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2019_jth_dunster_interspecies.pdf (1.5 MB)
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URI: http://hdl.handle.net/10347/21240
ISSN: 1538-7933
E-ISSN: 1538-7836
DOI: 10.1111/jth.14673

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2019

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Wiley
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Accurate protein quantification is a vital prerequisite for generating meaningful predictions when using systems biology approaches, a method that is increasingly being used to unravel the complexities of subcellular interactions and as part of the drug discovery process. Quantitative proteomics, flow cytometry, and western blotting have been extensively used to define human platelet protein copy numbers, yet for mouse platelets, a model widely used for platelet research, evidence is largely limited to a single proteomic dataset in which the total amount of proteins was generally comparatively higher than those found in human platelets.

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GPVI| Mathematical modelling| Platelet| Syk

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Dunster, JL, Unsworth, AJ, Bye, AP, et al. Interspecies differences in protein expression do not impact the spatiotemporal regulation of glycoprotein VI mediated activation. J Thromb Haemost. 2020; 18: 485– 496. https://doi.org/10.1111/jth.14673

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This work was supported by the Academy of Medical Science springboard grant to A.Y.P. [SBF002\1099]. The support of the British Heart Foundation (BHF) is gratefully acknowledged by J.L.D., (PG/16/20/32074) T.S., A.P.B and A.J.U (RG/15/2/31224) to J.M.G. B.N. is supported by the Deutsche Forschungsgemeinschaft (grant SFB/TR 240). The mouse model was generated with support from the BHF (FS/15/71/31677). E.J.H. was supported by a BHF grant (RG/13/18/30563) to S.P.W. who holds a BHF (CH0/003) supporting Y.D and N.S.P. M.S. is supported by a European Union’s Horizon 2020 research and innovation program under Marie Sklodowska‐Curie grant agreement No. 766118 to S.P.W., N.S.P., A.Y.P., J.M.G., B.N., and A.G

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© 2019 The Authors and the Journal of Thrombosis and Haemostasis. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited

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Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica
Centro de Investigación en Medicina Molecular e Enfermidades Crónicas (CiMUS)