RT Journal Article
T1 Interspecies differences in protein expression do not impact the spatiotemporal regulation of glycoprotein VI mediated activation
A1 Dunster, Joanne L.
A1 Unsworth, Amanda J.
A1 Bye, Alexander P.
A1 Haining, Elizabeth J.
A1 Sowa, Marcin A.
A1 Di, Ying
A1 Sage, Tanya
A1 Pallin, Chiara
A1 Pike, Jeremy A.
A1 Hardy, Alexander T.
A1 Nieswandt, Bernhard
A1 García Alonso, Ángel
A1 Watson, Steve P.
A1 Poulter, Natalie S.
A1 Gibbins, Jonathan M.
A1 Pollitt, Alice Y.
K1 GPVI
K1 Mathematical modelling
K1 Platelet
K1 Syk
AB Accurate protein quantification is a vital prerequisite for generating meaningful predictions when using systems biology approaches, a method that is increasingly being used to unravel the complexities of subcellular interactions and as part of the drug discovery process. Quantitative proteomics, flow cytometry, and western blotting have been extensively used to define human platelet protein copy numbers, yet for mouse platelets, a model widely used for platelet research, evidence is largely limited to a single proteomic dataset in which the total amount of proteins was generally comparatively higher than those found in human platelets.
PB Wiley
SN 1538-7933
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21240
UL http://hdl.handle.net/10347/21240
LA eng
NO Dunster, JL, Unsworth, AJ, Bye, AP, et al. Interspecies differences in protein expression do not impact the spatiotemporal regulation of glycoprotein VI mediated activation. J Thromb Haemost. 2020; 18: 485– 496. https://doi.org/10.1111/jth.14673
NO This work was supported by the Academy of Medical Science springboard grant to A.Y.P. [SBF002\1099]. The support of the British Heart Foundation (BHF) is gratefully acknowledged by J.L.D., (PG/16/20/32074) T.S., A.P.B and A.J.U (RG/15/2/31224) to J.M.G. B.N. is supported by the Deutsche Forschungsgemeinschaft (grant SFB/TR 240). The mouse model was generated with support from the BHF (FS/15/71/31677). E.J.H. was supported by a BHF grant (RG/13/18/30563) to S.P.W. who holds a BHF (CH0/003) supporting Y.D and N.S.P. M.S. is supported by a European Union’s Horizon 2020 research and innovation program under Marie Sklodowska‐Curie grant agreement No. 766118 to S.P.W., N.S.P., A.Y.P., J.M.G., B.N., and A.G
DS Minerva
RD 24 abr 2026