A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

Thumbnail Image

Files

2016_chemsci_rodriguez_designed_DNA.pdf (1.24 MB)
Identifiers
URI: http://hdl.handle.net/10347/16016
ISSN: 2041-6539
DOI: 10.1039/c6sc00045b

Publication date

2016-02-05

Authors

Advisors

Tutors

Editors

Journal Title

Journal ISSN

Volume Title

Publisher

Royal Society of Chemistry
Metrics
Google Scholar
lacobus
Export

Research Projects

Organizational Units

Journal Issue

Abstract

We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG)

Description

Keywords

Bibliographic citation

J. Rodríguez, J. Mosquera, R. García-Fandiño, M. E. Vázquez and J. L. Mascareñas, Chem. Sci., 2016, 7, 3298–3303

Relation

Has part

Has version

Is based on

Is part of

Is referenced by

Is version of

Requires

Sponsors

We are thankful for the support given by the Spanish grants SAF2013-41943-R, CTQ2013-43264-R and CTQ2013-49317-EXP, the Xunta de Galicia GRC2013-041, the ERDF, and the European Research Council (Advanced Grant No. 340055). Support of COST Action CM1105, COST CM1306 and the orfeo-cinqa network are kindly acknowledged

Rights

© The author(s) 2016. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence

Collections

Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS)
Química Orgánica