RT Journal Article
T1 A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves
A1 Rodríguez Villar, Jéssica
A1 Mosquera Mosquera, Jesús
A1 García Fandiño, Rebeca
A1 Vázquez Sentís, Marco Eugenio
A1 Mascareñas Cid, José Luis
AB We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG)
PB Royal Society of Chemistry
SN 2041-6539
YR 2016
FD 2016-02-05
LK http://hdl.handle.net/10347/16016
UL http://hdl.handle.net/10347/16016
LA eng
NO J. Rodríguez, J. Mosquera, R. García-Fandiño, M. E. Vázquez and J. L. Mascareñas, Chem. Sci., 2016, 7, 3298–3303
NO We are thankful for the support given by the Spanish grantsSAF2013-41943-R, CTQ2013-43264-R and CTQ2013-49317-EXP,the Xunta de Galicia GRC2013-041, the ERDF, and the EuropeanResearch Council (Advanced Grant No. 340055). Support ofCOST Action CM1105, COST CM1306 and the orfeo-cinqanetwork are kindly acknowledged
DS Minerva
RD 28 abr 2026