A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

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dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Molecularesgl
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánicagl
dc.contributor.authorRodríguez Villar, Jéssica
dc.contributor.authorMosquera Mosquera, Jesús
dc.contributor.authorGarcía Fandiño, Rebeca
dc.contributor.authorVázquez Sentís, Marco Eugenio
dc.contributor.authorMascareñas Cid, José Luis
dc.date.accessioned2017-10-21T13:12:05Z
dc.date.available2017-10-21T13:12:05Z
dc.date.issued2016-02-05
dc.description.abstractWe report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG)gl
dc.description.peerreviewedSIgl
dc.description.sponsorshipWe are thankful for the support given by the Spanish grants SAF2013-41943-R, CTQ2013-43264-R and CTQ2013-49317-EXP, the Xunta de Galicia GRC2013-041, the ERDF, and the European Research Council (Advanced Grant No. 340055). Support of COST Action CM1105, COST CM1306 and the orfeo-cinqa network are kindly acknowledgedgl
dc.identifier.citationJ. Rodríguez, J. Mosquera, R. García-Fandiño, M. E. Vázquez and J. L. Mascareñas, Chem. Sci., 2016, 7, 3298–3303gl
dc.identifier.doi10.1039/c6sc00045b
dc.identifier.issn2041-6539
dc.identifier.urihttp://hdl.handle.net/10347/16016
dc.language.isoenggl
dc.publisherRoyal Society of Chemistrygl
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/340055
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2013-49317/ES/EXPLORACION DE TERAPIAS ANTITUMORALES ALTERNATIVAS DIRIGIDAS A LAS MITOCONDRIAS
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2013-43264-R/ES/NUEVAS TERAPIAS SUPRAMOLECULARES DIRIGIDAS A LAS MEMBRANAS LIPIDICAS Y BASADAS EN NANOESTRUCTURAS AUTOENSAMBLADAS
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2013-41943-R/ES/HERRAMIENTAS SINTETICAS EN QUIMICA BIOLOGICA. NUEVAS ESTRATEGIAS MOLECULARES PARA EL TRATAMIENTO Y DIAGNOSTICO DE CANCERES
dc.relation.publisherversionhttp://dx.doi.org/10.1039/C6SC00045Bgl
dc.rights© The author(s) 2016. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licencegl
dc.rights.accessRightsopen accessgl
dc.titleA designed DNA binding motif that recognizes extended sites and spans two adjacent major groovesgl
dc.typejournal articlegl
dc.type.hasVersionVoRgl
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery1b4c7a3c-215b-42bf-9ce2-c533031318dc

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