YY1 Positively Regulates Transcription by Targeting Promoters and Super-Enhancers through the BAF Complex in Embryonic Stem Cells

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dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicasgl
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Moleculargl
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Fisioloxíagl
dc.contributor.authorWang, Jia
dc.contributor.authorWu, Xingui
dc.contributor.authorWei, Chao
dc.contributor.authorHuang, Xin
dc.contributor.authorMa, Qian
dc.contributor.authorHuang, Xiaona
dc.contributor.authorFabiola, Francesco
dc.contributor.authorGuallar Artal, Diana
dc.contributor.authorFidalgo Pérez, Miguel Ángel
dc.contributor.authorHuang, Tingyuan
dc.contributor.authorPeng, Di
dc.contributor.authorChen, Li
dc.contributor.authorYu, Haopeng
dc.contributor.authorLi, Xingyu
dc.contributor.authorSun, Junyi
dc.contributor.authorLiu, Xinyi
dc.date.accessioned2020-05-28T16:09:44Z
dc.date.available2020-05-28T16:09:44Z
dc.date.issued2018
dc.description.abstractYin Yang 1 (YY1) regulates early embryogenesis and adult tissue formation. However, the role of YY1 in stem cell regulation remains unclear. YY1 has a Polycomb group (PcG) protein-dependent role in mammalian cells. The PcG-independent functions of YY1 are also reported, although their underlying mechanism is still undefined. This paper reports the role of YY1 and BAF complex in the OCT4-mediated pluripotency network in mouse embryonic stem cells (mESCs). The interaction between YY1 and BAF complex promotes mESC proliferation and pluripotency. Knockdown of Yy1 or Smarca4, the core component of the BAF complex, downregulates pluripotency markers and upregulates several differentiation markers. Moreover, YY1 enriches at both promoter and super-enhancer regions to stimulate transcription. Thus, this study elucidates the role of YY1 in regulating pluripotency through its interaction with OCT4 and the BAF complex and the role of BAF complex in integrating YY1 into the core pluripotency networkgl
dc.description.peerreviewedSIgl
dc.description.sponsorshipThis research was funded by grants from the National Key Research and Development Program (2016YFA [0101700] and 2017YFA0102800), the National Natural Science Foundation of China (31771639), the Guangdong Innovative and Entrepreneurial Research Team Program 2016ZT06S029, the Fundamental Research Funds for the Central Universities (17ykzd04), and Thousand Youth Talents Plan to J.D., the National Natural Science Foundation of China (81703086) to J.W. and the NIH (1R01-GM095942 and 1R21HD087722) and the Empire State Stem Cell funded through the New York State Department of Health (NYSTEM; C028103 and C028121) to J.W.gl
dc.identifier.citationWang,J., Wu, X., Wei, C., Huang, X., Ma, Q., Huang, X. et al. (2018). YY1 positively regulates transcription by targeting promoters and super-enhancers through the BAF complex in embryonic stem cells. Stem Cell Reports, vol. 10, 1324-1339gl
dc.identifier.doi10.1016/j.stemcr.2018.02.004
dc.identifier.essn2213-6711
dc.identifier.urihttp://hdl.handle.net/10347/22631
dc.language.isoenggl
dc.publisherCell Pressgl
dc.relation.publisherversionhttps://doi.org/10.1016/j.stemcr.2018.02.004gl
dc.rights© 2018 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)gl
dc.rights.accessRightsopen accessgl
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPolycomb groupgl
dc.subjectProtein-dependentgl
dc.subjectKnockdown of Yy1 or Smarca4gl
dc.subjectYY1gl
dc.subjectPcG-independentgl
dc.titleYY1 Positively Regulates Transcription by Targeting Promoters and Super-Enhancers through the BAF Complex in Embryonic Stem Cellsgl
dc.typejournal articlegl
dc.type.hasVersionVoRgl
dspace.entity.typePublication
relation.isAuthorOfPublicationd94656ad-ed33-4a6c-9407-fc1d84b41289
relation.isAuthorOfPublicationc2e8c7dd-5b10-4a18-916d-eed1c5831aff
relation.isAuthorOfPublication.latestForDiscoveryd94656ad-ed33-4a6c-9407-fc1d84b41289

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