A Folding-Based Approach for the Luminescent Detection of a Short RNA Hairpin

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2013_jamchemsoc_penas_folding.pdf (2.02 MB)
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URI: http://hdl.handle.net/10347/18479
ISSN: 0002-7863
E-ISSN: 1520-5126
DOI: 10.1021/ja400270a

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2013

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American Chemical Society
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Abstract

We report the rational design of a 20-mer basic peptide, derived from the transcriptional antitermination protein N of bacteriophage P22, equipped with a luminescent DOTA[Tb3+] macrocyclic complex and a sensitizing tryptophan antenna. Folding of this peptide into an α helical conformation, which occurs upon binding to its target boxB RNA hairpin, results in a large increase in luminescence emission. Therefore, the peptide construct works as a highly sensitive and selective probe for this RNA hairpin

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This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Biology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see: https://pubs.acs.org/doi/10.1021/ja400270a

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Penas, C., Pazos, E., Mascareñas, J., & Vázquez, M. (2013). A Folding-Based Approach for the Luminescent Detection of a Short RNA Hairpin. J. Am. Chem. Soc., 135, 10, 3812–3814. doi: 10.1021/ja400270a

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We thank the financial support provided by the Spanish grants SAF2010-20822-C02, CTQ2012-31341, CSD2007-00006, Consolider Ingenio 2010, the Xunta de Galicia GRC2010/12, INCITE09 209084PR, and PGIDIT08CSA-047209PR. C.P. thanks the Spanish Ministerio de Educacion for her Ph.D. fellowship, and E.P. thanks the Xunta de Galicia for her postdoctoral contract

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© 2013 American Chemical Society

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Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS)
Química Orgánica