Integrity and quantity of salivary cell-free DNA as a potential molecular biomarker in oral cancer: a preliminary study

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dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Cirurxía e Especialidades Médico-Cirúrxicasgl
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Psiquiatría, Radioloxía, Saúde Pública, Enfermaría e Medicinagl
dc.contributor.authorRapado González, Óscar
dc.contributor.authorLópez Cedrún, José Luis
dc.contributor.authorLago Lestón, Ramón
dc.contributor.authorAbalo Piñeiro, Alicia
dc.contributor.authorRubin Roger, Guillermo
dc.contributor.authorSalgado Barreira, Ángel
dc.contributor.authorLópez López, Rafael
dc.contributor.authorMuinelo Romay, Laura
dc.contributor.authorSuárez Cunqueiro, María Mercedes
dc.date.accessioned2022-09-21T12:45:00Z
dc.date.available2022-09-21T12:45:00Z
dc.date.issued2022
dc.description.peerreviewedSIgl
dc.description.sponsorshipBackground: differences in cell-free DNA (cfDNA) fragments have been described as a valuable tool to distinguish cancer patients from healthy individuals. We aim to investigate the concentration and integrity of cfDNA fragments in saliva from oral squamous cell carcinoma (OSCC) patients and healthy individuals in order to explore their value as diagnostic biomarkers. Methods: saliva samples were collected from a total of 34 subjects (19 OSCC patients and 15 healthy controls). The total concentration of salivary cfDNA (scfDNA) was determined using a fluorometry method and quantitative real-time polymerase chain reaction (qPCR). To evaluate the scfDNA quantity and integrity, qPCR targeting Arthobacter luteus (ALU) sequences at three amplicons of different lengths (60, 115, and 247 bp, respectively) was carried out. ScfDNA integrity indexes (ALU115/ALU60 and ALU247/ALU60) were calculated as the ratio between the absolute concentration of the longer amplicons 115 bp and 247 bp and the total scfDNA amount (amplicon 60 bp).Results: the total scfDNA concentration (ALU60) was higher in OSCC than in healthy donors, but this trend was not statistically significant. The medians of scfDNA integrity indexes, ALU115/ALU60 and ALU247/ALU60, were significantly higher in OSCC, showing area under the curve values of 0.8211 and 0.7018, respectively. Conclusion: our preliminary results suggest that scfDNA integrity indexes (ALU115/ALU60 and ALU247/ALU60) have potential as noninvasive diagnostic biomarkers for OSCCgl
dc.identifier.citationJ Oral Pathol Med.2022;51:429–435gl
dc.identifier.doi10.1111/jop.13299
dc.identifier.essn1600-0714
dc.identifier.issn0904-2512
dc.identifier.urihttp://hdl.handle.net/10347/29238
dc.language.isoenggl
dc.publisherWileygl
dc.relation.publisherversionhttps://doi.org/10.1111/jop.13299gl
dc.rights© 2022 The Authors. Journal of Oral Pathology & Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly citedgl
dc.rightsAtribución 4.0 Internacional
dc.rights.accessRightsopen accessgl
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectALU repeatgl
dc.subjectDNA integritygl
dc.subjectLiquid biopsygl
dc.subjectOral Squamous Cell Carcinoma (OSCC)gl
dc.subjectSalivagl
dc.subjectSalivary cell-free DNA (scfDNA)gl
dc.titleIntegrity and quantity of salivary cell-free DNA as a potential molecular biomarker in oral cancer: a preliminary studygl
dc.typejournal articlegl
dc.type.hasVersionVoRgl
dspace.entity.typePublication
relation.isAuthorOfPublication980a4649-5d9a-4ba4-9f77-5bc5895aeb6e
relation.isAuthorOfPublication379cc913-eaca-4c1b-a99a-6e686435238d
relation.isAuthorOfPublication192571e0-bfb5-41d1-a68c-568dbde0a7ef
relation.isAuthorOfPublication.latestForDiscovery980a4649-5d9a-4ba4-9f77-5bc5895aeb6e

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Cirurxía e Especialidades Médico-Cirúrxicas
Psiquiatría, Radioloxía, Saúde Pública, Enfermaría e Medicina