High prevalence of quorum-sensing and quorum-quenching activity among cultivable bacteria and metagenomic sequences in the Mediterranean sea
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Abstract
There is increasing evidence being accumulated regarding the importance of N-acyl
homoserine lactones (AHL)-mediated quorum-sensing (QS) and quorum-quenching (QQ) processes
in the marine environment, but in most cases, data has been obtained from specific microhabitats,
and subsequently little is known regarding these activities in free-living marine bacteria. The QS and
QQ activities among 605 bacterial isolates obtained at 90 and 2000 m depths in the Mediterranean Sea
were analyzed. Additionally, putative QS and QQ sequences were searched in metagenomic data
obtained at different depths (15–2000 m) at the same sampling site. The number of AHL producers
was higher in the 90 m sample (37.66%) than in the 2000 m sample (4.01%). However, the presence
of QQ enzymatic activity was 1.63-fold higher in the 2000 m sample. The analysis of putative QQ
enzymes in the metagenomes supports the relevance of QQ processes in the deepest samples, found
in cultivable bacteria. Despite the unavoidable biases in the cultivation methods and biosensor
assays and the possible promiscuous activity of the QQ enzymes retrieved in the metagenomic
analysis, the results indicate that AHL-related QS and QQ processes could be common activity in the
marine environment
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Muras, A.; López-Pérez, M.; Mayer, C.; Parga, A.; Amaro-Blanco, J.; Otero, A. High Prevalence of Quorum-Sensing and Quorum-Quenching Activity among Cultivable Bacteria and Metagenomic Sequences in the Mediterranean Sea. Genes 2018, 9, 100.
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https://doi.org/10.3390/genes9020100Sponsors
This work was supported by the non-profit organizations Fundación Ramón Areces
(CIVP16A1814), the EU Project Byefouling (grant agreement no 612717), and by the grant “Axudas do
Programa de Consolidación e Estructuración de Unidades de Investigación Competitivas (GPC)” from the
Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia (ED431B2017/53). A.M. was
supported by a predoctoral fellowship from the Consellería de Cultura, Educación e Ordenación Universitaria,
Xunta de Galicia (ED481A-2015/311). M.L.P. was supported by a Postdoctoral fellowship from the Valencian
Consellería de Educació, Investigació, Cultura i Esport (APOSTD/2016/051). We would like to thank
Paul Williams from University of Nottingham and Tomohiro Morohoshi from Utsunomiya University for kindly
providing us with the Chromobacterium violaceum CV026 and VIR07 biosensor strains, respectively
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© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)