RT Journal Article
T1 High prevalence of quorum-sensing and quorum-quenching activity among cultivable bacteria and metagenomic sequences in the Mediterranean sea
A1 Muras Mora, Andrea
A1 López Pérez, Mario
A1 Mayer Mayer, Celia
A1 Parga Martínez, Ana
A1 Amaro Blanco, Jaime
A1 Otero Casal, Ana María
K1 Quorum sensing
K1 Quorum quenching
K1 AHL
K1 Lactonase
K1 Acylase
K1 Marine bacteria
AB There is increasing evidence being accumulated regarding the importance of N-acylhomoserine lactones (AHL)-mediated quorum-sensing (QS) and quorum-quenching (QQ) processesin the marine environment, but in most cases, data has been obtained from specific microhabitats,and subsequently little is known regarding these activities in free-living marine bacteria. The QS andQQ activities among 605 bacterial isolates obtained at 90 and 2000 m depths in the Mediterranean Seawere analyzed. Additionally, putative QS and QQ sequences were searched in metagenomic dataobtained at different depths (15–2000 m) at the same sampling site. The number of AHL producerswas higher in the 90 m sample (37.66%) than in the 2000 m sample (4.01%). However, the presenceof QQ enzymatic activity was 1.63-fold higher in the 2000 m sample. The analysis of putative QQenzymes in the metagenomes supports the relevance of QQ processes in the deepest samples, foundin cultivable bacteria. Despite the unavoidable biases in the cultivation methods and biosensorassays and the possible promiscuous activity of the QQ enzymes retrieved in the metagenomicanalysis, the results indicate that AHL-related QS and QQ processes could be common activity in themarine environment
PB MDPI
YR 2018
FD 2018
LK http://hdl.handle.net/10347/22949
UL http://hdl.handle.net/10347/22949
LA eng
NO Muras, A.; López-Pérez, M.; Mayer, C.; Parga, A.; Amaro-Blanco, J.; Otero, A. High Prevalence of Quorum-Sensing and Quorum-Quenching Activity among Cultivable Bacteria and Metagenomic Sequences in the Mediterranean Sea. Genes 2018, 9, 100.
NO This work was supported by the non-profit organizations Fundación Ramón Areces(CIVP16A1814), the EU Project Byefouling (grant agreement no 612717), and by the grant “Axudas doPrograma de Consolidación e Estructuración de Unidades de Investigación Competitivas (GPC)” from theConsellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia (ED431B2017/53). A.M. wassupported by a predoctoral fellowship from the Consellería de Cultura, Educación e Ordenación Universitaria,Xunta de Galicia (ED481A-2015/311). M.L.P. was supported by a Postdoctoral fellowship from the ValencianConsellería de Educació, Investigació, Cultura i Esport (APOSTD/2016/051). We would like to thankPaul Williams from University of Nottingham and Tomohiro Morohoshi from Utsunomiya University for kindlyproviding us with the Chromobacterium violaceum CV026 and VIR07 biosensor strains, respectively
DS Minerva
RD 25 abr 2026