Zeng, QiHu, BeijuanBlanco Hortas, AndrésZhang, WanchangZhao, DaxianHong, YijiangMartínez Portela, Paulino2024-01-312024-01-312022Zeng, Q., Hu, B., Blanco, A. H., Zhang, W., Zhao, D., Martínez, P., & Hong, Y. (2022). Full-length transcriptome sequences provide insight into hermaphroditism of freshwater pearl mussel Hyriopsis schlegelii. Frontiers in Genetics, 13, 868742. https://doi.org/10.3389/fgene.2022.868742http://hdl.handle.net/10347/32159The freshwater mussel Hyriopsis schlegelii is a cultured bivalve in China, and the quality of the pearls produced is affected by the type of gonads. However, because of the lack of a published genome and the complexity of sex determination, research on sex reversal and development of this species is limited. In this study, Illumina RNA-seq and PacBio Isoform Sequencing (Iso-Seq) were combined to analyze the gonads of H. schlegelii. A total of 201,481 high-quality transcripts were generated. The study identified 7,922 differentially expressed genes in three comparison group (females versus males, hermaphrodites versus females, and hermaphrodites versus males). Twenty-four genes were identified as potential sex-related genes, including sox9 and wnt4 involved in sex determination, and vtg, cyp17a1 and 17β-hsd2 involved in gonadal development. We also speculated a possible pathways for the formation of hermaphroditism in H. schlegelii. The data provide a clear view of the transcriptome for H. schlegelii gonads and will be valuable in elucidating the mechanisms of gonad developmentengCC-BYhttp://creativecommons.org/licenses/by-nc-sa/4.0/Hyriopsis schlegeliiIso-seqRNA-seqGonad17β-hsd2journal article10.3389/fgene.2022.8687421664-8021open access