Bandín Matos, IsabelSouto Pereira, SandraCutrín, J. M.López Vázquez, CarmenOlveira Hermida, José G.Esteve Sánchez, ConsueloAlcaide Moreno, ElenaPereira Dopazo, Carlos2026-02-202026-02-202014-05-20Bandín I, Souto S, Cutrín JM, López-Vázquez C, Olveira JG, Esteve C, Alcaide E, Dopazo CP. Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain). J Fish Dis. 2014 Jul;37(7):597-607. doi: 10.1111/jfd.1392. Epub 2014 May 20. PMID: 24846700https://hdl.handle.net/10347/46010This is the peer reviewed version of the following article: Bandín I, Souto S, Cutrín JM, López-Vázquez C, Olveira JG, Esteve C, Alcaide E, Dopazo CP. Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain). J Fish Dis. 2014 Jul;37(7):597-607. doi: 10.1111/jfd.1392, which has been published in final form at https://doi.org/10.1111/jfd.1392. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibitedA virological analysis was conducted on wild eels from the Albufera Lake (Spain). A total of 179 individuals at different growth stages were collected in two different surveys (2004 and 2008). Presence of anguillid herpesvirus (AngHV-1), aquabirnavirus and betanodavirus was confirmed by PCR procedures in both surveys, although the number of detections was clearly higher in 2008 (83% of the eels analysed resulted positive for virus presence). AngHV-1 was the viral agent most frequently detected, followed by aquabirnaviruses. Betanodaviruses were detected by the first time in wild eels, and although the detections were only made by nested PCR, high percentage of positives were achieved. In addition, in 2008, seven aquabirnaviruses were isolated. Phylogenetic analysis performed using partial sequences of both genomic segments of aquabirnaviruses indicated that the seven isolates could be typed as WB (genogroup I) on the basis of segment A sequences, but when segment B was used six of them clustered with C1 strain (genogroup V) and one was typed as Ab (genogroup II). These results indicate natural reassortment between different strains of aquabirnaviruses in the eels. Although betanodaviruses were not isolated in cell culture, the analysis of the sequence of the nested PCR product indicated that they clustered with SJNNV genotype. The diversity of viral agents and the high level of viral detections suggest that viral infections may play a more prominent role in the decline of the European eel than initially thoughtengAnguilla anguillaAnguillid herpesvirusAquabirnavirusesBetanodavirusesWild eels24 Ciencias de la vidajournal article10.1111/jfd.13921365-2761open access