Brea López, DavidAgulla Freire, JesúsRodríguez Yáñez, ManuelBarral, DavidRamos Cabrer, PedroCampos Pérez, FranciscoAlmeida, ÁngelesDávalos, AntoniCastillo Sánchez, José Antonio2020-04-292020-04-292014Brea, D., Agulla, J., Rodríguez‐Yáñez, M., Barral, D., Ramos‐Cabrer, P., Campos, F., Almeida, A., Dávalos, A. and Castillo, J. (2014), Regulatory T cells modulate inflammation and reduce infarct volume in experimental brain ischaemia. J. Cell. Mol. Med., 18: 1571-1579. doi:10.1111/jcmm.12304http://hdl.handle.net/10347/21865Brain ischaemia (stroke) triggers an intense inflammatory response predominately mediated by the accumulation of inflammatory cells and mediators in the ischaemic brain. In this context, regulatory T (Treg) cells, a subpopulation of CD4+ T cells with immunosuppressive and anti‐inflammatory properties, are activated in the late stages of the disease. To date, the potential therapeutic usefulness of Treg cells has not been tested. In this study, we aimed to investigate whether Treg cells exert protection/repair following stroke. Both the adoptive transfer of Treg cells into ischaemic rats and the stimulation of endogenous T‐cell proliferation using a CD28 superagonist reduced the infarct size at 3–28 days following the ischaemic insult. Moreover, T cell‐treated animals had higher levels of FoxP3 and lower levels of IL‐1β, CD11b+ and CD68+ cells in the infarcted hemisphere when compared with control animals. However, T‐cell treatment did not alter the rate of proliferation of NeuN‐, NCAM‐ or CD31‐positive cells, thereby ruling out neurogenesis and angiogenesis in protection. These results suggest that adoptive transfer of T cells is a promising therapeutic strategy against the neurological consequences of stroke.eng© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly citedhttps://creativecommons.org/licenses/by/3.0/Cerebrovascular disease/strokeRegulatory T cellsInflammationImmunomodulationjournal article10.1111/jcmm.123041582-4934open access