Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus

Heras, SandraPlanella, LaiaCaldarazzo, IlariaVera Rodríguez, ManuelGarcía Marín, José LuisRoldán, María Inés2021-05-042021-05-042016Heras S, Planella L, Caldarazzo I, Vera M, García-Marín J, Roldán MI. 2016. Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus. PeerJ 4:e2200 https://doi.org/10.7717/peerj.2200http://hdl.handle.net/10347/26111The blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishing grounds. Potential microsatellite markers (97) identified from next-generation sequencing of an individual shrimp using a 454 GS Junior Pyrosequencer were tested on a preliminary panel of 15 individuals representing the four worldwide genetic stocks of the species from which 35 polymorphic loci were identified and used to characterize an additional 20 individuals from the Western Mediterranean Sea. In the Western Mediterranean sample, 32 out of 35 were polymorphic loci and the number of alleles per locus ranged from 2 to 14 and expected heterozygosity ranged from 0.050 to 0.968. No linkage disequilibrium was detected, indicating the independence of the loci. These novel microsatellites provide additional tools to address questions relating to genetic diversity, parentage studies and connectivity patterns of A. antennatus populations and help develop effective strategies to ensure long-term sustainability of this resourceeng© 2016 Heras et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be citedAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Aristeus antennatusNext-generation sequencingMicrosatellite lociPopulation geneticsGenetic stocksBlue and red shrimpDevelopment and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatusjournal article10.7717/peerj.22002167-8359open access