Nieto Fontarigo, Juan JoséGonzález Barcala, Francisco JavierAndrade-Bulos, Luis JuanSan-José, María EstherCruz, María JesúsValdés Cuadrado, LuisCrujeiras Casais, Rosa MaríaArias Crespo, María del PilarSalgado Castro, Francisco Javier2025-01-302025-01-302020-12-01Nieto-Fontarigo JJ, González-Barcala FJ, Andrade-Bulos LJ, et al. iTRAQ-based proteomic analysis reveals potential serum biomarkers of allergic and nonallergic asthma. Allergy. 2020;75:3171–3183. https://doi.org/10.1111/ all.144060105-4538https://hdl.handle.net/10347/39340This is the peer reviewed version of the following article: Nieto-Fontarigo JJ, González-Barcala FJ, Andrade-Bulos LJ, et al. iTRAQ-based proteomic analysis reveals potential serum biomarkers of allergic and nonallergic asthma. Allergy. 2020;75:3171–3183, which has been published in final form at https://doi.org/10.1111/ all.14406. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.Background: Asthma is heterogeneous disease with different phenotypes, endotypes and severities. Definition of these subgroups requires the identification of biomarkers in biological samples, and serum proteomics is a useful and minimally invasive method for this purpose. Therefore, the aim of this study was to detect serum proteins whose abundance is distinctively associated with different asthma phenotypes (allergic vs nonallergic) or severities. Methods: For each group of donors (32 healthy controls, 43 allergic rhinitis patients and 192 asthmatics with different phenotypes and severities), we generated two pools of sera that were analysed by a shotgun MS approach based on combinatorial peptide ligand libraries and iTRAQ-LC-MS/MS. Results: MS analyses identified 18 proteins with a differential abundance. Functional/network study of these proteins identified key processes for asthma pathogenesis, such as complement activation, extracellular matrix organization, platelet activation and degranulation, or post-translational protein phosphorylation. Furthermore, our results highlighted an enrichment of the “Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)” route in allergic asthma and the lectin pathway of complement activation in nonallergic asthma.engAllergic asthmaAsthma biomarkerIGFALSNonallergic asthmaSerum proteomics32 Ciencias médicasjournal article10.1111/all.144061398-9995open access