Arias Baño, AdriánAllegue Martínez, Tomás FranciscoDarwich, AlaaFachal, ManuelMartínez Quintela, MiguelOmil Prieto, FranciscoGarrido Fernández, Juan Manuel2022-08-302022-08-302022Chemical Engineering Journal 450 (2022) 138289http://hdl.handle.net/10347/29173The use of N-damo bacteria, which can simultaneously remove nitrite and dissolved methane, could represent a cost-effective and sustainable alternative to minimize the environmental impact of effluents from methanogenic reactors treating domestic sewage. The operation of N-damo bacteria reactors is often not stable, and their activity decrease with no proven reason over time. This study aims to optimize the nitrite removal capacity of a lab-scale MBR by using different operating strategies for 878 days. The reactor was operated in continuous mode and with biomass highly enriched in these microbes. It was found that adjustments in the mineral medium concentration (reduction of Ca, P, and K) and composition (EDTA addition, increasing the Cu and Fe bioavailability), contributed to achieving remarkable and prolonged nitrite removal rates of up to 285.7 mg NO2–-N/L/d. N-damo bacteria dominated the culture, up to 57 %. To the best of our knowledge, the rates attained are the highest reported in an N-damo bacteria enrichmenteng© 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by- nc-nd/4.0/)Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Anaerobic methane oxidationN-damo bacteriaNitrogen removalTrace elementsMacronutrientsMBRjournal article10.1016/j.cej.2022.1382891385-8947open access