Lamas Freire, AlexandreRegal López, PatriciaVázquez Belda, Beatriz IsabelCepeda Sáez, AlbertoFranco Abuín, Carlos Manuel2018-01-042018-01-042017-12Lamas, A., Regal, P., Vázquez, B., Cepeda, A., Franco, C.M., (2017). Development of a transcriptomic protocol combining bovine somatic cells and OpenArray® technology to trace the ab(use) of somatotropin in dairy cattle. “Journal of Integrated Omics, a methodological journal”, vol. 7 (Special Issue: Book of Abstracts ISPROF 2017), 202182-0287http://hdl.handle.net/10347/16237SPECIAL ISSUE: SELECTED ABSTRACTS OF THE III INTERNATIONAL CAPARICA SYMPOSIUM ON PROFILING (ISPROF 2017),Costa de Caparica, Portugal, 4th-7th September 2017In the case of recombinant bovine somatotropin (rbST), in Europe it is key to control misuse in cattle in vivo, during the period of lactation of the cow. Therefore, target samples have to be easy to collect, the method of collection should be non-invasive and it has to be economically viable. The goal of this study was to develop a transcriptomic protocol based in the combination of High-Throughput Real-Time PCR and milk somatic cells to detect the ab(use) of rbST in dairy cattle. The results showed that MSCs are an optimal alternative to isolate RNA with good quality using a simple protocol for their collection. The use of OpenArray® technology made possible to establish a transcriptomic profile of treated and control cows that could be used to detect the illegal use of rBST in dairy cattle in EUeng© 2017 Proteomass Scientific Society. This work is licensed under a Creative Commons Attribution 3.0 Licensehttps://creativecommons.org/licenses/by/3.0/SomatotropinSomatic cellMilkTranscriptomicMaterias::Investigación::33 Ciencias tecnológicas::3309 Tecnología de los alimentos::330915 Higiene de los alimentosjournal article10.5584/jiomics.v7i2.212open access