Rodríguez Villamayor, PaulaRobledo Sánchez, DiegoFernández López, CarlosGullón Álvarez, JuliánQuintela Arias, Luis ÁngelSánchez Quinteiro, PabloMartínez Portela, Paulino2022-03-172022-03-172021Genomics 113 (2021) 2240-2252. https://doi.org/10.1016/j.ygeno.2021.05.007http://hdl.handle.net/10347/27675The vomeronasal organ (VNO) is a chemosensory organ specialized in pheromone detection that shows a broad morphofunctional and genomic diversity among mammals. However, its expression patterns have only been well-characterized in mice. Here, we provide the first comprehensive RNA sequencing study of the rabbit VNO across gender and sexual maturation stages. We characterized the VNO transcriptome, updating the number and expression of the two main vomeronasal receptor families, including 128 V1Rs and 67 V2Rs. Further, we defined the expression of formyl-peptide receptor and transient receptor potential channel families, both known to have specific roles in the VNO. Several sex hormone-related pathways were consistently enriched in the VNO, highlighting the relevance of this organ in reproduction. Moreover, whereas juvenile and adult VNOs showed significant transcriptome differences, male and female did not. Overall, these results contribute to understand the genomic basis of behavioural responses mediated by the VNO in a non-rodent modeleng© 2021 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)http://creativecommons.org/licenses/by-nc-nd/4.0/Vomeronasal organGene expressionAgeSexVNO-receptorsRabbitsRNA-Seqjournal article10.1016/j.ygeno.2021.05.0070888-7543open access