Pérez Fernández, RománSeoane Ruzo, SamuelMartínez Ordóñez, Anxo2019-10-072019-10-072019http://hdl.handle.net/10347/19865The POU1F1 transcription factor (also known Pit-1) is expressed in the mammary gland and its overexpression induces profound phenotypic changes in proteins involved in breast cancer progression. However, some mediators of Pit-1 pro-tumoral actions are still unknown. Here, we show that CXCR4 receptor and its ligand CXCL12 play a critical role in the pro-tumoral process induced by Pit-1. We found that Pit-1 increases the expression of CXCR4 and CXCL12. Knock-down of CXCR4 reduces tumor growth and spread of Pit-1 overexpressing cells in a zebrafish xenograft model. Furthermore, we described pro-angiogenic effects of Pit-1 through the CXCL12-CXCR4 axis, and that extravasation of Pit-1 overexpressing breast cancer cells is strongly reduced in CXCL12-deprived target tissues. Finally, in breast cancer patients, expression of Pit-1 in primary tumors was found to be positively correlated with CXCR4 and CXCL12, with specific metastasis in liver and lung, and with clinical outcome. Our results suggest that Pit-1-CXCL12-CXCR4 axis could be involved in chemotaxis guidance during the metastatic process, and may represent prognostic and/or therapeutic targets in breast tumors.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/BreastCellCancerMaterias::Investigación::24 Ciencias de la vida::2410 Biología humana::241010 Fisiología humanaMaterias::Investigación::24 Ciencias de la vida::2411 Fisiología humana::241104 Fisiología endocrinadoctoral thesisopen access