González Jartín, Jesús MaríaRodríguez Cañás, InésAlvariño Romero, RebecaAlfonso Rancaño, María AmparoSáinz Oses, María JesúsRodríguez Vieytes, MercedesGomes, AnaRamos, IsabelBotana López, Luis Miguel2024-09-242024-09-242024-06-28Food Control 165 (2024) 1106820956-7135http://hdl.handle.net/10347/34857This study aims to explore the presence of mycotoxins in total mixed rations (TMR) employed for feeding dairy cattle and their potential carry-over to milk. A total of 87 TRM samples were collected in farms from the north of Portugal from 2019 to 2022. A method based on a QuEChERS extraction followed by UHPLC-MS/MS detection was employed for sample analysis. The method was in-house validated in terms of matrix effect, linearity, sensitivity, accuracy and precision. The most frequently detected regulated toxins were fumonisins, with a positivity rate of 74%, while deoxynivalenol and zearalenone were found in approximately 35% of the samples. Among the emerging toxins, beauvericin and enniatins exhibited the highest detection rates. In addition, milk samples were collected from 21 farms, providing insights into the carry-over of these toxins and roquefortine to milk, with an estimated rate ranging between 2% and 10%engAttribution-NonCommercial-NoDerivatives 4.0 Internacional© 2024 The Authors. Published by Elsevier Ltd. This article is available under the Creative Commons CC-BY-NC-ND licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/MycotoxinsQuEChERSTotal mixed rationTransferenceUHPLC-MS/MSjournal article10.1016/j.foodcont.2024.1106821873-7129open access