Rodríguez Villar, JéssicaLearte Aymamí, SorayaMosquera Mosquera, JesúsCelaya, GarbiñeRodríguez Larrea, DavidVázquez Sentís, Marco EugenioMascareñas Cid, José Luis2020-05-262020-05-262018Rodríguez, J., Learte-Aymamí, S., Mosquera, J., Celaya, G., Rodríguez-Larrea, D., Vázquez, M., & Mascareñas, J. (2018). DNA-binding miniproteins based on zinc fingers. Assessment of the interaction using nanopores. Chemical Science, 9(17), 4118-4123. https://doi.org/10.1039/c7sc05441f2041-6520http://hdl.handle.net/10347/22612Obtaining artificial proteins that mimic the DNA binding properties of natural transcription factors could open new ways of manipulating gene expression at will. In this context it is particularly interesting to develop simple synthetic systems. Inspired by the modularity of natural transcription factors, we have designed synthetic miniproteins that combine the zinc finger module of the transcription factor GAGA and AT-hook peptide domains. These constructs are capable of binding to composite DNA sequences of up to 14 base pairs with high affinity and good selectivity. In particular, we have synthesized three different chimeras and characterized their DNA binding properties by electrophoresis and fluorescence anisotropy. We have also used, for the first time in the study of peptide-based DNA binders, nanopore force spectroscopy to obtain further data on the DNA interaction.eng© The Royal Society of Chemistry 2018. This article is licensed under a Creative Commons Attribution 3.0 Unported Licencehttps://creativecommons.org/licenses/by/3.0/journal article10.1039/c7sc05441f2041-6539open access