Carballido Nogueira, AroaArias Calvo, AnaFeijoo Costa, GumersindoMoreira Vilar, María Teresa2026-01-092026-01-092026-01-01Industrial Crops and Products Volume 240, February 2026, 1226250926-6690https://hdl.handle.net/10347/44978The growing demand for sustainable alternatives to petrochemical-based plastics has positioned 2,5-furandicarboxylic acid (FDCA) as a promising monomer for next-generation bioplastics like polyethylene furanoate (PEF). Among the available routes, enzymatic oxidation of 5-hydroxymethylfurfural (HMF) using 5-hydroxymethylfurfural oxidase (HMFO) stands out for its selectivity and mild operational conditions. This study aims to assess the sustainability potential of FDCA production via HMFO (including also the analysis of the enzyme production), integrating both techno-economic analysis (TEA) and life cycle assessment (LCA) methodology. Four fermentation volumes were evaluated for the HMFO production (5 L–1 m³), showing that the environmental loads decrease with scale-up, being the 1 m3 scenario the most promising one under an environmental perspective. Regarding the main contributors of the environmental profiles, glucose and cellulose are the process inputs that stand out, while renewable energy and reduced solvent input showed significant improvements in lowering burdens. Regarding the FDCA production, it was modelled both at lab and scale-up levels, including ethanol recycling as a mitigation strategy. In this case, HMF was identified as the primary hotspot across all scenarios and impact categories. On the other hand, the TEA revealed that a minimum selling price of $33.56/kg is needed to ensure that the process is economically viable, with enzyme reuse offering significant potential for cost reduction. Even though the sustainability potential has been demonstrated, both environmentally and economically, further research is needed in terms of enzyme reuse and recovery.eng© 2026 The Authors. Published by Elsevier B.V. Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Enzymatic productionBiotechnologyLife cycle assessmentTechno-economic analysisModelling Bioplastics PEFjournal article10.1016/j.indcrop.2026.122625open access