Pazo Pascual, MartaJuanes Carrasco, MarisaLostalé Seijo, IreneMontenegro García, Javier2018-07-052018-11-232018-05-23Pazo, M., Juanes, M., Lostalé-Seijo, I., & Montenegro, J. (2018). Oligoalanine helical callipers for cell penetration. Chemical Communications, 54(50), 6919-6922. doi: 10.1039/c8cc02304b1359-7345http://hdl.handle.net/10347/16956This is the accepted manuscript of the following article: Pazo, M., Juanes, M., Lostalé-Seijo, I., & Montenegro, J. (2018). Oligoalanine helical callipers for cell penetration. Chem. Commun., 2018, 54, 6919-6922. doi: 10.1039/c8cc02304b. This article may be used for non-commercial purposes in accordance with RSC Terms and Conditions for self-archivingEven for short peptides that are enriched in basic amino acids, the large chemical space that can be spanned by combinations of natural amino acids hinders the rational design of cell penetrating peptides. We here report on short oligoalanine scaffolds for the fine-tuning of peptide helicity in different media and the study of cell penetrating properties. This strategy allowed the extraction of the structure/activity features required for maximal membrane interaction and cellular penetration at minimal toxicity. These results confirmed oligoalanine helical callipers as optimal scaffolds for the rational design and the identification of cell penetrating peptideseng© Royal Society of Chemistry 2018. This article may be used for non-commercial purposes in accordance with RSC Terms and Conditions for self-archivingjournal article10.1039/C8CC02304B1364-548Xopen access