Álvarez Villamarín, María ClaraCameselle Teijeiro, José ManuelRodrigues, Joana Sousa2020-02-042021-01-092019http://hdl.handle.net/10347/20670Anaplastic thyroid carcinoma (ATC) has a low incidence but is lethal, with rare cases of survival for more than a few months. There are no specific clinical trials nor effective therapies. Our group has established a system where thyroid cancer from patients is cultivated in a standardized way parallelly with their benign/normal samples in order to study specific differences from the cancer. Through differential proteomics between cancer and benign samples, a PIAS2 protein more expressed in thyroid cancer was discovered. An RNA interference (dsRNAi) specific for beta isoform was designed. PIAS2b dsRNAi induces death of anaplastic cells without affecting differentiated carcinoma cells. Through cellular and molecular biology techniques and an in vivo orthotopic PDX model it was demonstrated that PIAS2 is essential for the mitosis of the anaplastic cells and their reduction induces mitotic catastrophe. PIAS2b is a specific therapeutic target in ATC.spaAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/cáncer anaplásico de tiroidesRNA de interferencia dsRNAiproteómicacatástrofe mitóticaMaterias::Investigación::24 Ciencias de la vida::2411 Fisiología humana::241104 Fisiología endocrinaMaterias::Investigación::24 Ciencias de la vida::2407 Biología celular::240701 Cultivo celulardoctoral thesisopen access