Calo Lapido, RenataPenas Tallón, CristinaJiménez Balsa, AdriánVázquez Sentís, Marco EugenioMascareñas Cid, José Luis2020-02-202020-05-292019Calo-Lapido, R., Penas, C., Jiménez-Balsa, A., Vázquez, M. E., Mascareñas, J. L. (2019), A chemical approach for the synthesis of the DNA-binding domain of the oncoprotein MYC. Org. Biomol. Chem., 17: 6748-6752. doi:10.1039/C9OB01209Ehttp://hdl.handle.net/10347/20792This is the peer reviewed version of the following article: Calo-Lapido, R., Penas, C., Jiménez-Balsa, A., Vázquez, M. E., Mascareñas, J. L. (2019), A chemical approach for the synthesis of the DNA-binding domain of the oncoprotein MYC. Org. Biomol. Chem., 17: 6748-6752, which has been published in final form at https://doi.org/10.1039/C9OB01209E. This article may be used for non-commercial purposes in accordance with RSC Terms and Conditions for Use of Self-Archived VersionsWe describe the first chemical synthesis of a functional mutant of the DNA binding domain of the oncoprotein MYC, using two alternative strategies which involve either one or two Native Chemical Ligations (NCLs). Both routes allowed the efficient synthesis of a miniprotein which is capable of heterodimerizing with MAX, and replicate the DNA binding of the native protein. The versatility of the reported synthetic approach enabled the straightforward preparation of MYC and Omomyc analogues, as well as fluorescently labeled derivativeseng© The Royal Society of Chemistry 2019. This article may be used for non- commercial purposes in accordance with RSC Terms and Conditions for Use of Self-Archived Versionsjournal article10.1039/C9OB01209E1477-0539open access