Juanes Carrasco, MarisaLostalé Seijo, IreneGranja Guillán, Juan RamónMontenegro García, Javier2018-05-022018-10-272018-04-27Juanes, M., Lostalé-Seijo, I., Granja, J., & Montenegro, J. (2018). Supramolecular Recognition and Selective Protein Uptake by Peptide Hybrids. Chemistry - A European Journal. http://dx.doi.org/10.1002/chem.201800706http://hdl.handle.net/10347/16690This manuscript has been accepted after peer review and appears as anAccepted Article online prior to editing, proofing, and formal publicationof the final Version of Record (VoR). This work is currently citable byusing the Digital Object Identifier (DOI) given below. The VoR will bepublished online in Early View as soon as possible and may be differentto this Accepted Article as a result of editing. Readers should obtainthe VoR from the journal website shown below when it is publishedto ensure accuracy of information. The authors are responsible for thecontent of this Accepted ArticleThe intracellular transport of exogenous proteins has emerged as one of the most promising methodologies for biotechnology and chemical biology. Current protein delivery is mainly approached by liposome encapsulation, translational fusion and ionic/hydrophobic non‐covalent aggregation with transporting molecular vehicles. We here introduce the concept of supramolecular recognition and selective transport of proteins by peptide hybrid materials. We have designed a helical amphiphilic cationic peptide that bears two orthogonal alkoxyamines for the precise anchoring of protein ligands. After the attachment of these protein ligands, the peptide showed a high binding affinity for its protein target (i.e. mannose/Concanavalin A, Biotin/Streptavidin). The resulting peptide/protein hybrids were taken up by human cells such as HeLa and HepG2. The concept described in this manuscript could potentially be adapted, through the appropriate choice of ligands, to the transport of other proteins with suitable supramolecular binding motifseng© 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, WeinheimSupramolecular systemsMembranesPeptidesPenetrating peptidesAmphiphilesProteinTransportSupramolecular Recognition and Selective Protein Uptake by Peptide Hybridsjournal article10.1002/chem.2018007061521-3765open access