Martínez Matamoros, DianaCastro García, SocorroBalado Dacosta, MiguelMatamoros Veloza, AdrianaCamargo Valero, Miller AlonsoCespedes, OscarRodríguez, JaimeJiménez, CarlosLemos Ramos, Manuel Luis2020-07-132020-07-132019Martínez Matamoros, D., Castro García, S., Balado Dacosta, M., Matamoros Veloza, A., Camargo Valero, M.A., Cespedes, O. et al. (2019). Preparation of functionalized magnetic nanoparticles conjugated with feroxamine and their evaluation for pathogen detection. RSC Advances, vol. 9, 13533-135422046-2069http://hdl.handle.net/10347/23119This work reports the preparation of a conjugate between amino-functionalized silica magnetite and thesiderophore feroxamine. The morphology and properties of the conjugate and intermediate magneticnanoparticles (MNPs) were examined by powder X-ray diffraction (XRD), Fourier Transform Infraredspectroscopy (FT-IR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), magnetizationstudies, zeta potential measurements, Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray (EDX) mapping. Furthermore, this study investigated the interaction between the functionalizedmagnetic NPs andYersinia enterocoliticawild type (WC-A) using Scanning Electron Microscopy (SEM)and TEM images. In addition, the interaction between MNPs and aY. enterocoliticamutant strain lackingferoxamine receptor FoxA, was also used to study the binding specificity. The results showed that thecapture and isolation ofY. enterocoliticaby the MNPs took place in all cases. Moreover, the specificinteraction between the MNP conjugate and bacteria did not increase after blocking the free aminegroups witht-butoxycarbonyl (Boc) and carboxylic acid (COOH) functional groups. Electrostatic surfaceinteractions instead of molecular recognition between MNP conjugate and feroxamine receptor seem torule the attachment of bacteria to the conjugateeng© The Royal Society of Chemistry 2019. Open Access Article. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licencehttp://creativecommons.org/licenses/by-nc/3.0/NanoparticlesFeroxaminePathogenjournal article10.1039/c8ra10440aopen access