Moscoso, IsabelFernandez-Trasancos, ÁngelCabro, MaríaCouselo, MarinelaFandiño-Vaquero, RubénBravo, Susana B.Sierra, JuanEiras, SoniaLage Fernández, RicardoGonzález Juanatey, José Ramón2025-01-222025-01-222015-11-15Ricardo Lage, Isabel Moscoso, Ángel Fernández-Trasancos, María Cebro, Marinela Couselo, Rubén Fandiño-Vaquero, Susana B. Bravo, Juan Sierra, José Ramón González-Juanatey, Sonia Eiras, Differential behaviour of epicardial adipose tissue-secretomes with high and low orosomucoid levels from patients with cardiovascular disease in H9C2 cells, Molecular and Cellular Endocrinology, Volume 416, 2015, Pages 77-87, ISSN 0303-7207, https://doi.org/10.1016/j.mce.2015.08.025.0303-7207https://hdl.handle.net/10347/38872Epicardial adipose tissue releases orosomucoid (ORM), an acute phase protein with multiple modulatory and protective properties. We aimed to identify the effect of EAT-supernatants according to their ORM levels on H9C2 cells. H9C2 were cultured with EAT-secretomes or ORM protein itself on a Real-Time Cell Analyser. Secretome proteins identification was performed by LC-mass spectrometry according to their ORM levels. Two of them were validated by ELISA in EAT-supernatants from 42 patients. ORM effect on H9C2 and neonatal rat cardiomyocytes apoptosis under hypoxia with or without fatty acid treatment was determined by Annexin-V flow cytometry measurement. Caspase-3 expression levels were determined by western blot in H9C2. Our results showed a differential effect of EAT-secretomes according their ORM levels. Although additional secreted proteins can contribute to their beneficial effects, ORM reduced hypoxia-induced apoptosis through caspase-3 inhibition. Our data showed the cardioprotective role of ORM and suggest that its quantification on EAT secretomes might help us to find new secreted factors with a cardioprotective role.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Epicardial fatSecretomeOrosomucoidH9C2 cellsjournal article10.1016/j.mce.2015.08.0251872-8057open access