Martínez Sernández, VictoriaMuiño Pose, LauraPerteguer, María JesúsGárate, TeresaMezo, MercedesGonzález Warleta, MartaMuro, AntonioCosta, José Manuel Correia daRomarís Martínez, FernandaMartínez Ubeira, Florencio César2020-05-052020-05-052011Martínez-Sernández V., Muíño L., Perteguer M.J., Gárate T., Mezo M., et al. (2011). Development and Evaluation of a New Lateral Flow Immunoassay for Serodiagnosis of Human Fasciolosis. PLoS Negl Trop Dis 5(11): e1376. https://doi.org/10.1371/journal.pntd.00013761935-2727http://hdl.handle.net/10347/22036Background: Human fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F. hepatica and F. gigantica). Human fasciolosis can be diagnosed by classical coprological techniques, such as the Kato-Katz test, to reveal parasite eggs in faeces. However, although 100% specific, these methods are generally not adequate for detection of acute infections, ectopic infections, or infections with low number of parasites. In such cases immunological methods may be a good alternative and are recommended for use in major hospitals where trained personnel are available, although they are not usually implemented for individual testing. Methodology/Principal Findings: We have developed a new lateral flow test (SeroFluke) for the serodiagnosis of human fasciolosis. The new test was constructed with a recombinant cathepsin L1 from F. hepatica, and uses protein A and mAb MM3 as detector reagents in the test and control lines, respectively. In comparison with an ELISA test (MM3-SERO) the SeroFluke test showed maximal specificity and sensitivity and can be used with serum or whole blood samples. Conclusions/Significance: The new test can be used in major hospitals in hypoendemic countries as well as in endemic/ hyperendemic regions where point-of-care testing is required.eng© 2011 Martínez-Sernández et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedhttps://creativecommons.org/licenses/by/2.0/journal article10.1371/journal.pntd.0001376open access