Remuñán López, CarmenFernández Paz, Estefanía2022-04-202023-03-302022http://hdl.handle.net/10347/28134Gene therapy offers hope of cure for rare and other serious diseases that currently have no established effective treatments. It has become a valuable therapeutic strategy for congenital or acquired genetic lung diseases, such as alpha1-antitrypsin deficiency, infections and some types of lung cancer, among others. In this Thesis work, a new pulmonary delivery platform consisting of mannitol microspheres (Ma MS) containing chitosan-based nanocapsules (CS-based NCs) loaded with the model plasmid pCMV-betaGal is proposed for its direct delivery to the lung. The designed micro-nanostructures have suitable aerodynamic properties, are biocompatible with the A549 lung cell line and are distributed in the deep lung, producing transfection.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Chitosan nanocapsulesdry powdersmicroencapsulationpCMV-betaGalpulmonary gene deliveryspray-dryingMaterias::Investigación::32 Ciencias médicas::3209 Farmacología::320908 Preparación de medicamentosdoctoral thesisopen access