Rapado González, ÓscarBrea Iglesias, JeniferRodríguez Casanova, AitorBao Caamaño, AidaLópez Cedrún, José LuisTriana Martínez, GabrielDíaz Peña, RobertoArminda Santos, MaríaLópez López, RafaelMuinelo Romay, LauraMartínez Fernández, MónicaDíaz Lagares, ÁngelSuárez Cunqueiro, María Mercedes2025-01-202025-01-202022-11-24Rapado-González Ó, Brea-Iglesias J, Rodríguez-Casanova A, et al. Somatic mutations in tumor and plasma of locoregional recurrent and/or metastatic head and neck cancer using a next-generation sequencing panel: A preliminary study. Cancer Med. 2023;12:6615-6622. doi:10.1002/cam4.54362045-7634https://hdl.handle.net/10347/38745Background We explore the utility of TruSight Tumor 170 panel (TST170) for detecting somatic mutations in tumor and cfDNA from locoregional recurrent and/or metastatic head and neck squamous cell carcinoma (HNSCC). Methods Targeted NGS of tumor DNA and plasma cfDNA was performed using TST170 panel. In addition, a set of somatic mutations previously described in HNSCC were selected for validating in tumor, plasma, and saliva by digital droplet PCR. Results The TST170 panel identified 13 non-synonymous somatic mutations, of which five were detected in tumoral tissue, other five in plasma cfDNA, and three in both tissue and plasma cfDNA. Of the eight somatic mutations identified in tissue, three were also identified in plasma cfDNA, showing an overall concordance rate of 37.5%. Conclusions This preliminary study shows the possibility to detect somatic mutations in tumor and plasma of HNSCC patients using a single assay that would facilitate the clinical implementation of personalized medicine in the cliniceng© 2022 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution LicenseAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Cell-free DNADroplet digital PCRHead and neck squamous cell carcinomaLiquid biopsyNext-generation sequencingSalivaSomatic mutations32 Ciencias médicasjournal article10.1002/cam4.54362045-7634open access