Rial Pensado, EvaRivas Limeres, VerónicaGrijota Martínez, CarmenRodríguez Díaz, AmandaCapelli, VallentinaBarca Mayo, OlgaNogueiras Pozo, RubénMittag, JensDiéguez González, CarlosLópez Pérez, Miguel A.2025-01-182025-01-182022-11-18Rial Pensado, E., Rivas-Limeres, V., Grijota-Martínez, C., Rodríguez-Díaz, A., Capelli, C., Barca-Mayo, O., Nogueiras, R., Mittag, J., Diéguez, C., López, M. (2022). Temperature modulates systemic and central actions of thyroid hormones on BAT thermogenesis. "Frontiers in Physiology", vol. 13, 1017381.1664-042Xhttps://hdl.handle.net/10347/38692Thyroid hormones (THs) play a major role regulating energy balance and brown adipose tissue (BAT) thermogenesis, as well as body temperature, as shown in hyperthyroid patients. However, the current landscape of preclinical thyroid hormone models is complex. For example, while rats become catabolic after TH administration, mice gain weight; so, these differences in species need to be analyzed in detail and specially whether temperature could be a factor. Here, we aimed to investigate the effect of environmental temperature on those actions. Rats were subcutaneously treated with L-thyroxine (T4) or stereotaxically within the ventromedial nucleus of the hypothalamus (VMH) with triiodothyronine (T3) and housed at 23°C, 4°C or 30°C; energy balance, BAT thermogenesis and AMP-activated protein kinase (AMPK) in the VMH were analyzed. Our data showed that the effect of both systemic T4 of central T3 on energy balance and BAT thermogenesis was dependent upon environmental temperature. This evidence is of interest in the design of experimental settings highlighting the species-specific metabolic actions of THs, and in understanding its physiological role in the adaptation to temperature.eng© 2022 Rial-Pensado, Rivas-Limeres, Grijota-Martínez, Rodríguez-Díaz, Capelli, Barca-Mayo, Nogueiras, Mittag, Diéguez and López. This is an openaccess article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.http://creativecommons.org/licenses/by/4.0/Thyroid hormonesTemperatureThermogenesisBrown Adippose TissueHypothalamusAMPK241104 Fisiología endocrina241109 Regulación de la temperatura humanajournal article10.3389/fphys.2022.1017381open access