Lema Rodicio, Juan ManuelLu Chau, Thelmo AlejandroGarcía Torreiro, María2017-06-072017-06-072017http://hdl.handle.net/10347/15454The main aim of this Thesis is to develop processes that allow the integrated production of biopolymers into biorefineries, using feedstocks from first and second generation as raw materials. The basic nutritional requirements for growth and polyhydroxyalkanoate (PHA) accumulation with the bacterium Halomonas boliviensis were firstly determined. Besides, an analytical methodology based on flow cytometry was developed for a faster quantification of the PHA content. Two different substrates from first generation feedstocks, cereal and sugarcane juice, were evaluated as carbon source for PHA production by H. boliviensis. A novel process configuration was implemented and evaluated, based on the strategy of simultaneous saccharification and fermentation, for the PHA production from cereal mash. Finally, the utilization of volatile fatty acids, derived from residual streams, was evaluated as carbon source. This allowed to upgrade the biorefinery concept. To sum up, the results of this Thesis provide different routes for the formulation of processes that allow the integration of the biopolymers production into a biorefinery and, particularly, information about the general PHA accumulation capacity of H. boliviensis and its specific performance growing on complex carbon sources.engEsta obra atópase baixo unha licenza internacional Creative Commons BY-NC-ND 4.0. Calquera forma de reprodución, distribución, comunicación pública ou transformación desta obra non incluída na licenza Creative Commons BY-NC-ND 4.0 só pode ser realizada coa autorización expresa dos titulares, salvo excepción prevista pola lei. Pode acceder Vde. ao texto completo da licenza nesta ligazón: https://creativecommons.org/licenses/by-nc-nd/4.0/deed.glhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.glbiorefinerypolyhydroxyalkanoatesHalomonas boliviensisMaterias::Investigación::33 Ciencias tecnológicas::3302 Tecnología bioquímica::330202 Tecnología de la fermentaciónMaterias::Investigación::24 Ciencias de la vida::2414 Microbiología::241403 Metabolismo bacterianodoctoral thesisopen access