Barca Mayo, OlgaCarneiro, CarmenCostoya Puente, José AntonioSeñarís Rodríguez, Rosa MaríaArce Vázquez, Víctor Manuel2025-01-212025-01-212010-05-18Barca-Mayo, O., Carneiro, C., Costoya, J.A., Señarís, R.M., Arce, V.M. (2010). Resistance of neonatal primary astrocytes against Fas-induced apoptosis depends on silencing of caspase 8. "Neuroscience Letters", vol. 479(3), 206-210.0304-3940https://hdl.handle.net/10347/38853In the present report, we have found that primary fetal astrocytes express caspase 8 and undergo apoptosis in response to Fas ligation. In contrast, neonatal astrocytes do not express detectable levels of the enzyme and are resistant to Fas killing. Fas-induced apoptosis can be restored in these cells by up-regulation of caspase 8 expression by means of transient transfection with a caspase 8-encoding plasmid. Furthermore, treatment of primary astrocytes with the demethylating agent 5-Aza-dC restores caspase 8 expression and increases the sensibility of neonatal astrocytes to the cytotoxic effect of Fas activation. Altogether, our findings indicate that silencing of caspase 8 gene is a key factor controlling the outcome of neonatal astrocytes upon Fas engagement.engAstrocytesFasApoptosisCaspase-8Epigenetic241111 Neurofisiologíajournal article10.1016/j.neulet.2010.05.057restricted access