Alvariño Romero, RebecaAlonso López, EvaAlfonso Rancaño, María AmparoBotana López, Luis Miguel2024-01-082024-01-082019-12-14Alvariño, R., Alonso, E., Alfonso, A., & Botana, L. M. (2020). Neuroprotective Effects of Apple-Derived Drinks in a Mice Model of Inflammation. Molecular nutrition & food research, 64(2), e1901017http://hdl.handle.net/10347/31771This is the peer reviewed version of the following article: Alvariño, R., Alonso, E., Alfonso, A., & Botana, L. M. (2020). Neuroprotective Effects of Apple-Derived Drinks in a Mice Model of Inflammation. Molecular nutrition & food research, 64(2), e1901017, which has been published in final form at https://doi.org/10.1002/mnfr.201901017. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.Scope Fruit-derived drinks consumption is considered beneficial due to the antioxidant and neuroprotective effects of polyphenols separately, but studies including their total constituents are scarce. In this work, the antioxidant and anti-inflammatory neuroprotective effects of apple-derived beverages are determined in a mouse model of lipopolysaccharide (LPS)-induced inflammation. Methods and results Preliminary antioxidant and neuroinflammatory experiments are carried out with 15 drink polyphenolic extracts in SH-SY5Y and BV2 cells, using H2O2 as pro-oxidant and LPS as pro-inflammatory stimulus, respectively. Extracts improve antioxidant systems functioning and present neuroprotective mitochondrial-related effects. In microglia, extracts reduce reactive oxygen species and modulate cytokine release. To better mimic human consumption, four concentrated dealcoholized apple-derived drinks (three ciders and apple juice) are supplied to mice for 7 days in substitution of drinking water. Mice treated with beverages present reduced brain oxidative stress and inflammatory markers after LPS injection. Interestingly, genetic expression of antioxidant enzymes and glutathione levels are also greatly augmented after drink intake. Conclusion The results confirm the antioxidant and anti-inflammatory-mediated neuroprotective properties of apple-derived drinks, suggesting that their consumption could be a good approach for prevention of neurodegenerative disorders. To the authors’ knowledge, this is the first description of cider neuroprotective effects.engAnti-inflammationApple juiceCiderNeuroprotectionOxidative stressjournal article10.1002/mnfr.2019010171613-4133open access