Alonso Fernández, María JoséLópez Borrajo, Mireya2024-02-052023http://hdl.handle.net/10347/32336Genetic therapies show great potential for treating various diseases through modulation of the expression of specific genes. Their effective delivery is crucial, particularly for challenging conditions like CNS diseases and SARS-CoV-2 vaccines. Here, we used nanotechnology as a fundamental tool for RNA delivery. First, we engineered an ionizable nanoemulsion designed for brain diffusion and delivery of RNA. Second, we explored nanocarriers loaded with siRNA for brain delivery, exploring their performance following alternative administration routes. Finally, we encapsulated mRNAs derived from SARS-CoV-2 within nanoemulsions and polymeric nanocapsules, achieving significant cellular immune responses.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/nanotechnologygenetic medicineRNAcentral nervous system diseasesvaccines320802 Acción de los medicamentos241210 Vacunasdoctoral thesisopen access