Montes Goyanes, RosaRodil Rodríguez, María del RosarioPlacer Vidal, LorenaWilms, Jonas M.Cela Torrijos, RafaelQuintana Álvarez, José Benito2021-04-272021-04-272020Analytical and Bioanalytical Chemistry (2020) 412:4849–48561618-2642http://hdl.handle.net/10347/26078This is a post-peer-review, pre-copyedit version of an article published in Analytical and Bioanalytical Chemistry. The final authenticated version is available online at: https://doi.org/10.1007/s00216-020-02434-wA new analytical method for the determination of 22 perfluoroalkylated (carboxylic and sulfonic) acids in water samples is presented. The method's objective was to achieve the simultaneous quantification of compounds with different chain lengths (from C1 to C18). To this end, 500 mL of water were extracted with Oasis WAX solid-phase extraction cartridges and eluted with 3 mL of 5% ammonia in methanol. After evaporation to dryness, extracts were reconstituted in methanol:ultrapure water (1:1) and analyzed by mixed-mode liquid chromatography-tandem mass spectrometry (MMLC-MS/MS) using a weak anion exchange/reversed-phase column. The method provided good results, with limits of quantification lower than 1 ng/L in river water for most of compounds, except the two perfluorocarboxylic acids with the longest alkyl chain (>C14) and trifluoroacetic acid, for which a blank contamination problem was observed. The method proved good trueness and precision in both ultrapure and river water (R ≥ 81%, RSD ≤ 15%). After validation, the method was applied to the analysis of nine water samples where nine perfluoroalkylated acids were quantified. Seven of them were ultrashort- (C1-C4) and short-chain (C4-C8) perfluoroalkylated acids, pointing out the importance of developing methods capable to target such substances for further monitoringeng© Springer-Verlag GmbH Germany, part of Springer Nature 2020. This article may be used for non-commercial purposes in accordance with Springer Verlag Terms and Conditions for self-archivingPerfluoroalkyl carboxylic acids (PFCAs)Perfluoroalkyl sulfonic acids (PFSAs)Persistent and mobile organic contaminants (PMOCs)Solid-phase extractionWater samplesjournal article10.1007/s00216-020-02434-w1618-2650open access