Penas Tallón, CristinaPazos Chantrero, ElenaMascareñas Cid, José LuisVázquez Sentís, Marco Eugenio2019-03-272019-03-272013Penas, C., Pazos, E., Mascareñas, J., & Vázquez, M. (2013). A Folding-Based Approach for the Luminescent Detection of a Short RNA Hairpin. J. Am. Chem. Soc., 135, 10, 3812–3814. doi: 10.1021/ja400270a0002-7863http://hdl.handle.net/10347/18479This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Biology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see: https://pubs.acs.org/doi/10.1021/ja400270aWe report the rational design of a 20-mer basic peptide, derived from the transcriptional antitermination protein N of bacteriophage P22, equipped with a luminescent DOTA[Tb3+] macrocyclic complex and a sensitizing tryptophan antenna. Folding of this peptide into an α helical conformation, which occurs upon binding to its target boxB RNA hairpin, results in a large increase in luminescence emission. Therefore, the peptide construct works as a highly sensitive and selective probe for this RNA hairpineng© 2013 American Chemical Societyjournal article10.1021/ja400270a1520-5126open access