Fuentes Iglesias, AlejandroGarcía Outeiral, VeraPardavila Paz, José ÁngelWang, JianlongFidalgo Pérez, Miguel ÁngelGuallar Artal, Diana2025-01-172025-01-172020-09-04Fuentes-Iglesias et al., An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro, STAR Protocols (2020), https://doi.org/10.1016/j.xpro.2020.100093https://hdl.handle.net/10347/38657RNA-binding proteins are key regulators of cell identity and function, which underscores the need for unbiased and versatile protocols to identify and characterize novel protein-RNA interactions. Here, we describe a simple and cost-effective in vitro RNA immunoprecipitation (iv-RIP) method to assess the direct or indirect RNA-binding ability of any protein of interest. The versatility of this method relies on the adaptability of the immunoprecipitation conditions and the choice of the RNA, which exponentially broadens the range of potential applicationseng© 2020 The Author(s). This is an open access article under the CC BY-NC-ND licenseAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Immunoprecipitation ProtocolProtein-RNA InteractionsRNA-binding proteinsin vitro RNA immunoprecipitationiv-RIP32 Ciencias médicasjournal article10.1016/j.xpro.2020.1000932666-1667open access