Alonso Fernández, María JoséTorres López, DoloresSantalices Ramos, Irene2018-12-272018-12-272018http://hdl.handle.net/10347/18026The main goal of this thesis has been the generation of knowledge towards the advancement of the oral administration of peptides. The specific objective has been to study how the composition and surface properties of lipid-based nanocarriers influence i) their capacity to load hydrosoluble peptides and ii) their ability to overcome the biological barriers associated to the oral modality of administration. Throughout this study, we developed a novel mixed nanosystem (nanoemulsion co-existing with micelles) able to load hydrophobically-modified insulin with a 100 % association efficiency. In brief, this nanosystem showed a great stability and miscibility in bio-relevant media, acceptable mucodiffusive properties and ability to interact and enter the intestinal cells, without relevant cytotoxic effects. Interestingly, the promising in vitro behavior of this insulin-loaded nanosystem was translated into a moderate hypoglycemic response (≈ 20−30 %) following intestinal administration to rats.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Oral peptide deliveryNanocapsulesNanoemulsionsMaterias::Investigación::32 Ciencias médicas::3209 Farmacología::320908 Preparación de medicamentosMaterias::Investigación::32 Ciencias médicas::3209 Farmacología::320903 Evaluación de medicamentosdoctoral thesisopen access