Lamas Fernández, JesúsLeiro Vidal, José ManuelSitjà-Bobadilla, AriadnaBron, James E.Wiegertjes, GeertPiazzon, M. Carla2026-01-082026-01-082021Lamas, J., Leiro, J.M. (2021). Philasterides dicentrarchi. In: A. Sitjà-Bobadilla et al. (eds.). Fish parasites. A Handbook of Protocols for their Isolation, Culture and Transmission. 5M Books. 9781789181333 (pp. 33-48)9781789181333https://hdl.handle.net/10347/44921In this book chapter, we describe the equipment and methodologies used to isolate and culture the fish parasite Philasterides dicentrarchi, as well as the freezing techniques employed to maintain the parasite for extended periods. We also outline the procedures for transmission, infection, and vaccine preparation.eng© 5m Books 2021. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without prior permission of the copyright holderPhilasterides dicentrarchiIsolationCultureTransmissionFreezing methodologybook partrestricted access