Bouzada Reboredo, DavidPaul, AnanyaSánchez López, Mateo IsidroDomínguez-Asenjo, BárbaraLence Quintana, Emilio JoséMelle-Franco, ManuelArdá, AnaBarreiro Piñeiro, NataliaPenas, CristinaGómez González, JacoboJiménez-Barbero, JesúsMoreno, JavierGonzález Bello, ConcepciónWilson, W. DavidMascareñas Cid, José LuisVázquez Sentís, Marco Eugenio2025-03-072025-03-072024Bouzada, D., Paul, A., Sánchez, M. I., Domínguez-Asenjo, B., Lence, E., Melle-Franco, M., Ardá, A., Barreiro-Piñeiro, N., Penas, C., Gómez-González, J., Jiménez-Barbero, J., Moreno, J., González-Bello, C., Wilson, W. D., Mascareñas, J. L., & Vázquez, M. E. (2024). High-affinity A/T-rich DNA binding with a dimeric bisbenzamidine. NAR Molecular Medicine, 1(4), Article ugae022 . https://doi.org/10.1093/narmme/ugae0222976-856Xhttps://hdl.handle.net/10347/40115A bisbenzamidine DNA binding agent can be easily dimerized by alkylation of its terminal amidine groups to afford an extended minor groove binder with over 20-fold enhanced DNA affinity toward extended A/T-rich sites. Nuclear magnetic resonance experiments in combination with molecular dynamics simulation studies provide structural insight into the insertion of this compound in the DNA minor groove, and antimicrobial assays demonstrate that the increased affinity translates into higher antileishmanial activity.engAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/Dimeric bisbenzamidineADNA/T2306 Química orgánicajournal articledoi.org/10.1093/narmme/ugae0222976-856Xopen access