Soprano, EnricaMigliavacca, MartinaLópez Ferreiro, MiriamPelaz García, BeatrizPolo Tobajas, EsterPino González de la Higuera, Pablo Alfonso del2023-10-232023-10-232023-06-07Journal of Colloid and Interface Science 648 (2023) 488–496. https://doi.org/10.1016/j.jcis.2023.06.0150021-9797http://hdl.handle.net/10347/31054A surface-engineered cell-derived nanocarrier was developed for efficient cytosolic delivery of encapsulated biologically active molecules inside living cells. Thus, a combination of aromatic-labeled and cationic lipids, instrumental in providing fusogenic properties, was intercalated into the biomimetic shell of self-assembled nanocarriers formed from cell membrane extracts. The nanocarriers were loaded, as a proof of concept, with either bisbenzimide molecules, a fluorescently labeled dextran polymer, the bicyclic heptapeptide phalloidin, fluorescently labeled polystyrene nanoparticles or a ribonucleoprotein complex (Cas9/sgRNA). The demonstrated nanocarrieŕs fusogenic behavior relies on the fusogen-like properties imparted by the intercalated exogenous lipids, which allows for circumventing lysosomal storage, thereby leading to efficient delivery into the cytosolic milieu where cargo regains functioneng©2023 The Author(s).Published by Elsevier Inc.This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/BiomimeticIntracellular deliveryNanocarrierFusogenicCell membrane coatingjournal article10.1016/j.jcis.2023.06.015open access