Castrillo Arias, Pedro AntonioVarela Dopico, CatuxaBermúdez, RobertoOndina Navarret, María PazQuiroga Berdeal, María Isabel2024-01-192024-01-192021Castrillo PA, Varela-Dopico C, Bermúdez R, Ondina P, Quiroga MI. Morphopathology and gill recovery of Atlantic salmon during the parasitic detachment of Margaritifera margaritifera. J Fish Dis. 2021;44:1101–1115http://hdl.handle.net/10347/31900Trátase da versión previa á revisión enviada a Journal of Fish Diseases do seguinte artigo: Castrillo, PA; Varela-Dopico, C.; Bermúdez, R.; Ondina, P.& Quiroga, M.I (2021) Morphopathology and gill recovery of Atlantic salmon during the parasitic detachment of Margaritifera margaritifera que foi revisada e publicada finalmente en https://onlinelibrary.wiley.com/doi/full/10.1111/jfd.13372. Este artigo pode ser utilizado con uso non comercial segundo os termos e condicións da editorial Wiley para a utilización en versións autoarquivadas. O artigo forma forma parte dun proxecto multidisciplinar levado a cabo entre os grupos de investigación da USC: GI-1707_GAPAVET (Anatomía patológica veterinaria) e GI2040_COPEMOL (Conservación de Peixes e Moluscos), no que se desenvolveu a tese de doutoramento de Pedro Antonio Castrillo Arias. This is the peer reviewed version of the following article: Castrillo, PA; Varela-Dopico, C.; Bermúdez, R.; Ondina, P.& Quiroga, M.I (2021) Morphopathology and gill recovery of Atlantic salmon during the parasitic detachment of Margaritifera margaritifera which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1111/jfd.13372. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. The article is part of a multidisciplinary project developed by the research groups of the USC: GI-1707_GAPAVET (Veterinary Pathological Anatomy) and GI2040_COPEMOL (Conservation of Fish and Molluscs), in which the doctoral thesis carried out by Pedro Antonio Castrillo Arias was developed.During the conservation aquaculture of the freshwater mussel Margaritifera margaritifera, fish health has become a concern due to the need of mussel larvae (glochidia) to parasitize the salmonid gills and metamorphose into juveniles. However, there is a lack of information about the impact on fish during the juvenile detachment and the subsequent gill healing. To evaluate the morphopathological changes and gill recovery after the parasitism of M. margaritifera, 51 Atlantic salmon fry (Salmo salar), infested with around 22 larvae/fish g, were necropsied during the synchronized detachment of the mussel juveniles, and gills were assessed by stereomicroscopy and by light and scanning electron microscopy. Salmon showed no clinical signs during the trial and gills recovered their normal morphology almost completely in a short time, suggesting a minimal impact on fish health after glochidiosis. In this sense, the non-erosive droplet detachment and the goblet cell hyperplasia favoured an effective gill remodelling mediated by apoptosis, polarization and cell shedding of the gill epithelia, providing insights to the defence, clearing and healing mechanisms of the gill. These morphopathological techniques could also be implemented to preserve fish welfare and to optimize the artificial breeding programmes of endangered freshwater mussels.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Fish pathologyFreshwater pearl musselGil healingGlochidiosisParasite detachmentSalmo salarjournal article10.1111/jfd.13372open access