Sanz Esporrín, JavierDi Raimondo, RiccardoVignoletti, FabioNúñez, JavierMuñoz Guzón, Fernando MaríaSanz, Mariano2021-08-042021-08-042021Sanz-Esporrin, J., Di Raimondo, R., Vignoletti, F., Núñez, J., Muñoz, F., & Sanz, M. (2021). De novo bone formation around implants with a surface based on a monolayer of multi-phosphonate molecules. An experimental in vivo investigation. Clinical Oral Implants Research, 00, 1– 12. https://doi.org/10.1111/clr.13803http://hdl.handle.net/10347/26690Objectives: The purpose of this experimental in vivo investigation was to evaluate the influence of modifying the implant surface by adding a monolayer of multi-phosphonate molecules on the de novo bone formation and osseointegration. Material and Methods: The study was designed as an animal preclinical trial with intra-animal control and two healing periods, 2 and 8 weeks, to compare implants with an identical macro-design but with two different surfaces. Eight female Beagle dogs participated in the study. Control implants had a moderately rough surface combining sandblasting and acid etching; test implants had an additional monophosphonate layer covalently bonded to titanium. Histologic and radiographic (micro-CT) outcome variables were evaluated. Results: The first bone-to-implant contact (fBIC) was located more coronally for the test implants at the first (0.065 mm (95% CI = −0.82, 0.60)) and second healing milestones (0.17 mm (95% CI = −0.9, 0.55)). Most coronal BIC of the test implants displayed a higher percentage of osseointegration, +6.33% and +13.38% after 2 and 8 weeks, respectively; however, the differences were not statistically significant. The micro-CT examination did not show any BIC difference. Conclusions: The monophosphonate layer coating demonstrated clinical, histological, and radiographic results similar to the control surface.eng© 2021 The Authors. Clinical Oral Implants Research published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are madeAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Animal modelDental implantsHistologyImplant surfaceOsseointegrationMonophosphonate layerWound chamberHistometric analysisMicro-CTjournal article10.1111/clr.138031600-0501open access